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histalon gravity column  (TaKaRa)


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    Structured Review

    TaKaRa histalon gravity column
    Histalon Gravity Column, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/histalon+gravity+column/HisTALON+Gravity+Columns/pm42104416-331-17-20
    Average 95 stars, based on 110 article reviews
    histalon gravity column - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Development of a Double-Antigen Sandwich ELISA for Oz Virus and a Seroepidemiological Survey in Wild Boars in Miyazaki, Japan
    Article Snippet: Recombinant E. coli cells were lysed in xTractor Buffer with Recombinant DNase I (TaKaRa Bio), and the supernatant was collected by centrifugation. .. His-tagged recombinant proteins were purified using a HisTalon gravity column (TaKaRa Bio), followed by PD-10 Desalting Columns (GE Healthcare, Chicago, IL, USA), according to the manufacturers’ protocols. .. The purified recombinant protein was concentrated using an Amicon Ultra Centrifugal Filter, 30 kDa MWCO (Merck, Darmstadt, Germany).

    Article Title: Arylsulfatase LvArs2 regulates glycosaminoglycan sulfation and modulates white spot syndrome virus burden in Litopenaeus vannamei
    Article Snippet: .. The recombinant protein of XP_027225959.1 (r-LvARS2) was expressed in Escherichia coli (BL21-T1R), purified using a HisTALON Gravity Column (Clontech), and desalted using a PD-10 Desalting Column (GE Healthcare). ..

    Article Title: Development of a Double-Antigen Sandwich ELISA for Oz Virus and a Seroepidemiological Survey in Wild Boars in Miyazaki, Japan
    Article Snippet: Recombinant E. coli cells were lysed in xTractor Buffer with Recombinant DNase I (TaKaRa Bio), and the supernatant was collected by centrifugation. .. His-tagged recombinant proteins were purified using a HisTalon gravity column (TaKaRa Bio), followed by PD-10 Desalting Columns (GE Healthcare, Chicago, IL, USA), according to the manufacturers’ protocols. .. The purified recombinant protein was concentrated using an Amicon Ultra Centrifugal Filter, 30 kDa MWCO (Merck, Darmstadt, Germany).

    Purification:

    Article Title: Development of a Double-Antigen Sandwich ELISA for Oz Virus and a Seroepidemiological Survey in Wild Boars in Miyazaki, Japan
    Article Snippet: Recombinant E. coli cells were lysed in xTractor Buffer with Recombinant DNase I (TaKaRa Bio), and the supernatant was collected by centrifugation. .. His-tagged recombinant proteins were purified using a HisTalon gravity column (TaKaRa Bio), followed by PD-10 Desalting Columns (GE Healthcare, Chicago, IL, USA), according to the manufacturers’ protocols. .. The purified recombinant protein was concentrated using an Amicon Ultra Centrifugal Filter, 30 kDa MWCO (Merck, Darmstadt, Germany).

    Article Title: Arylsulfatase LvArs2 regulates glycosaminoglycan sulfation and modulates white spot syndrome virus burden in Litopenaeus vannamei
    Article Snippet: .. The recombinant protein of XP_027225959.1 (r-LvARS2) was expressed in Escherichia coli (BL21-T1R), purified using a HisTALON Gravity Column (Clontech), and desalted using a PD-10 Desalting Column (GE Healthcare). ..

    Article Title: PpNAC transcription factors regulate ENDOPOLYGALACTURONASE alleles and control peach fruit texture and stone adhesion.
    Article Snippet: Fruit texture variation in peach (Prunus persica L. Batsch), especially softening accompanied by the melting phase, directly affects marketability and storage.. Two tandem endopolygalacturonase (endoPG) genes at the F–M locus, PGF and PGM, determine the melting or nonmelting trait, which is associated with the freestone or clingstone trait.. In this study, freestone melting flesh (FMF), clingstone melting flesh (CMF), and clingstone nonmelting flesh (CNMF) cultivars were sampled across various developmental and postharvest stages.

    Article Title: Development of a Double-Antigen Sandwich ELISA for Oz Virus and a Seroepidemiological Survey in Wild Boars in Miyazaki, Japan
    Article Snippet: Recombinant E. coli cells were lysed in xTractor Buffer with Recombinant DNase I (TaKaRa Bio), and the supernatant was collected by centrifugation. .. His-tagged recombinant proteins were purified using a HisTalon gravity column (TaKaRa Bio), followed by PD-10 Desalting Columns (GE Healthcare, Chicago, IL, USA), according to the manufacturers’ protocols. .. The purified recombinant protein was concentrated using an Amicon Ultra Centrifugal Filter, 30 kDa MWCO (Merck, Darmstadt, Germany).

    Article Title: A Prussian Blue Nanozyme-Adjuvanted Vaccine Presenting Phosphocholine Antigens for Induction of Immunotolerance in Inflammatory Bowel Disease.
    Article Snippet: .. After high-speed centrifugation for 10 min to remove the precipitate, the supernatant was loaded onto a HisTALON gravity column (Takara Bio, USA) for purification of the target protein. ..

    Article Title: A Prussian Blue Nanozyme‐Adjuvanted Vaccine Presenting Phosphocholine Antigens for Induction of Immunotolerance in Inflammatory Bowel Disease
    Article Snippet: .. After high‐speed centrifugation for 10 min to remove the precipitate, the supernatant was loaded onto a HisTALON gravity column (Takara Bio, USA) for purification of the target protein. ..

    Centrifugation:

    Article Title: Cold storage delays peach fruit softening via m 6 A reader PpYTHDFE1 liquid-liquid phase separation-mediated degradation of cell wall-loosening transcript PpEXP3.
    Article Snippet: .. After centrifugation at 10,000 g for 40 min at 4 °C, the supernatants were passed through a HisTALON Gravity Column (Takara, Japan). ..

    Article Title: Comprehensive engineering of novel glycosyltransferase for efficient, donor-promiscuous, and regioselective glycosylation of flavonoids
    Article Snippet: .. The clear supernatant was collected by centrifugation at 13,000 rpm for 20 min at 4°C and applied to the HisTALON gravity column (Takara, China) to purify the target proteins. .. The concentration and purity of UGT75AJ2 was analyzed by protein quantitative kit (Beyotime Biotechnology) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively.

    Article Title: A Prussian Blue Nanozyme-Adjuvanted Vaccine Presenting Phosphocholine Antigens for Induction of Immunotolerance in Inflammatory Bowel Disease.
    Article Snippet: .. After high-speed centrifugation for 10 min to remove the precipitate, the supernatant was loaded onto a HisTALON gravity column (Takara Bio, USA) for purification of the target protein. ..

    Article Title: A Prussian Blue Nanozyme‐Adjuvanted Vaccine Presenting Phosphocholine Antigens for Induction of Immunotolerance in Inflammatory Bowel Disease
    Article Snippet: .. After high‐speed centrifugation for 10 min to remove the precipitate, the supernatant was loaded onto a HisTALON gravity column (Takara Bio, USA) for purification of the target protein. ..

    Transformation Assay:

    Article Title: PpNAC transcription factors regulate ENDOPOLYGALACTURONASE alleles and control peach fruit texture and stone adhesion.
    Article Snippet: Fruit texture variation in peach (Prunus persica L. Batsch), especially softening accompanied by the melting phase, directly affects marketability and storage.. Two tandem endopolygalacturonase (endoPG) genes at the F–M locus, PGF and PGM, determine the melting or nonmelting trait, which is associated with the freestone or clingstone trait.. In this study, freestone melting flesh (FMF), clingstone melting flesh (CMF), and clingstone nonmelting flesh (CNMF) cultivars were sampled across various developmental and postharvest stages.

    Incubation:

    Article Title: PpNAC transcription factors regulate ENDOPOLYGALACTURONASE alleles and control peach fruit texture and stone adhesion.
    Article Snippet: Fruit texture variation in peach (Prunus persica L. Batsch), especially softening accompanied by the melting phase, directly affects marketability and storage.. Two tandem endopolygalacturonase (endoPG) genes at the F–M locus, PGF and PGM, determine the melting or nonmelting trait, which is associated with the freestone or clingstone trait.. In this study, freestone melting flesh (FMF), clingstone melting flesh (CMF), and clingstone nonmelting flesh (CNMF) cultivars were sampled across various developmental and postharvest stages.

    Protein Purification:

    Article Title: PpNAC transcription factors regulate ENDOPOLYGALACTURONASE alleles and control peach fruit texture and stone adhesion.
    Article Snippet: Fruit texture variation in peach (Prunus persica L. Batsch), especially softening accompanied by the melting phase, directly affects marketability and storage.. Two tandem endopolygalacturonase (endoPG) genes at the F–M locus, PGF and PGM, determine the melting or nonmelting trait, which is associated with the freestone or clingstone trait.. In this study, freestone melting flesh (FMF), clingstone melting flesh (CMF), and clingstone nonmelting flesh (CNMF) cultivars were sampled across various developmental and postharvest stages.



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